Sample Preparation for TEM analysis (Bulk metal, Powder sample, Brittle material)

1. Can biological samples be seen under TEM?
Note: Since liquid nitrogen is used near sample, the sample can be frozen to avoid any damage from electron beam (and also resist any evaporation).
2. The internal structure does not change during crushing of this material:
3. Usually a copper grid is used in TEM holder for:
4. The carbon coating on copper grid is used for:
5. Typically, what thickness of material is transparent to electrons (say at 200 keV)?